FACULTY OF ENGINEERING

Department of Genetics and Bioengineering

BME 420 | Course Introduction and Application Information

Course Name
PCR-based Molecular Diagnosis
Code
Semester
Theory
(hour/week)
Application/Lab
(hour/week)
Local Credits
ECTS
BME 420
Fall/Spring
2
2
3
5

Prerequisites
None
Course Language
English
Course Type
Elective
Course Level
First Cycle
Mode of Delivery -
Teaching Methods and Techniques of the Course -
Course Coordinator
Course Lecturer(s)
Assistant(s)
Course Objectives The objective of this course to introduce the basics of polymerase chain reaction. Throughout the course students learn how to prepare samples and set up PCR instruments and evaluation of the results of PCR technique in diagnosis of genetical or communicable diseases.
Learning Outcomes The students who succeeded in this course;
  • Describe the principles of Polymerase Chain Reaction (PCR)
  • Can separate and discuss different PCR methods
  • DNA can be isolated from biological samples
  • Can design primer and probe for PCR
  • Can write PCR protocol
  • And it can evaluate the results obtained from the PCR device.
Course Description The course covers the definition and history of polymerase chain reaction (PCR) method and how this method evolved through the years. The course covers principles of modern PCR methods and basic application of a PCR protocol starting from sample preparation to evaluation of the results obtained from PCR instruments. The course covers the introduction to common genetic and communicable diseases and PCR applications for their diagnosis in modern medicine.

 



Course Category

Core Courses
Major Area Courses
Supportive Courses
Media and Management Skills Courses
Transferable Skill Courses

 

WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES

Week Subjects Related Preparation
1 Introduction to Molecular Diagnosis Lecture Notes
2 Principles of Polymerase Chain Reaction (PCR) Lecture Notes
3 Reverse Transcriptase PCR Lecture Notes
4 Quantitative PCR Lecture Notes
5 Biological Samples and Isolation Methods Lecture Notes
6 Sample Evaluation Lecture Notes
7 Primer design Lecture Notes
8 Probe design Lecture Notes
9 Probe design Lecture Notes
10 Sample Preparation for PCR Lecture Notes
11 Setting Up PCR Protocol Lecture Notes
12 Post-PCR Melting Analysis Lecture Notes
13 Normalization Methods and Reporting Lecture Notes
14 PCR-based diagnosis of Genetic and Communicable Diseases Lecture Notes
15 Review and Lab Design for PCR Lecture Notes
16 Final Exam

 

Course Notes/Textbooks

Lecture Notes

Suggested Readings/Materials

 

EVALUATION SYSTEM

Semester Activities Number Weigthing
Participation
Laboratory / Application
5
30
Field Work
Quizzes / Studio Critiques
Portfolio
Homework / Assignments
4
30
Presentation / Jury
Project
Seminar / Workshop
Oral Exams
Midterm
Final Exam
1
40
Total

Weighting of Semester Activities on the Final Grade
9
70
Weighting of End-of-Semester Activities on the Final Grade
1
30
Total

ECTS / WORKLOAD TABLE

Semester Activities Number Duration (Hours) Workload
Theoretical Course Hours
(Including exam week: 16 x total hours)
16
2
32
Laboratory / Application Hours
(Including exam week: '.16.' x total hours)
16
2
32
Study Hours Out of Class
12
3
36
Field Work
0
Quizzes / Studio Critiques
0
Portfolio
0
Homework / Assignments
4
5
20
Presentation / Jury
0
Project
0
Seminar / Workshop
0
Oral Exam
0
Midterms
0
Final Exam
1
30
30
    Total
150

 

COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP

#
Program Competencies/Outcomes
* Contribution Level
1
2
3
4
5
1

To have adequate knowledge in Mathematics, Science and Genetics and Bioengineering; to be able to use theoretical and applied information in these areas on complex engineering problems.

2

To be able to identify, define, formulate, and solve complex Genetics and Bioengineering problems; to be able to select and apply proper analysis and modeling methods for this purpose.

3

To be able to design a complex system, process, device or product under realistic constraints and conditions, in such a way as to meet the requirements; to be able to apply modern design methods for this purpose.

4

To be able to devise, select, and use modern techniques and tools needed for analysis and solution of complex problems in Genetics and Bioengineering applications; to be able to use information technologies effectively.

5

To be able to design and conduct experiments, gather data, analyze and interpret results for investigating complex engineering problems or Genetics and Bioengineering research topics.

6

To be able to work efficiently in Genetics and Bioengineering disciplinary and multi-disciplinary teams; to be able to work individually.

7

To be able to communicate effectively in Turkish, both orally and in writing; to be able to author and comprehend written reports, to be able to prepare design and implementation reports, to present effectively, to be able to give and receive clear and comprehensible instructions.

8

To have knowledge about global and social impact of Genetics and Bioengineering practices on health, environment, and safety; to have knowledge about contemporary issues as they pertain to engineering; to be aware of the legal ramifications of Genetics and Bioengineering solutions.

9

To be aware of ethical behavior, professional and ethical responsibility; to have knowledge about standards utilized in Genetics and Bioengineering applications.

10

To have knowledge about industrial practices such as project management, risk management, and change management; to have awareness of entrepreneurship and innovation; to have knowledge about sustainable development.

11

To be able to collect data in the area of Genetics and Bioengineering, and to be able to communicate with colleagues in a foreign language.

12

To be able to speak a second foreign language at a medium level of fluency efficiently.

13

To recognize the need for lifelong learning; to be able to access information, to be able to stay current with developments in science and technology; to be able to relate the knowledge accumulated throughout the human history to Genetics and Bioengineering.

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest

 


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