FACULTY OF ENGINEERING
Department of Genetics and Bioengineering
GBE 309 | Course Introduction and Application Information
| Course Name |
Genetic Engineering Techniques
|
|
Code
|
Semester
|
Theory
(hour/week) |
Application/Lab
(hour/week) |
Local Credits
|
ECTS
|
|
GBE 309
|
Fall
|
3
|
2
|
4
|
6
|
| Prerequisites |
|
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| Course Language |
English
|
|||||||||
| Course Type |
Required
|
|||||||||
| Course Level |
First Cycle
|
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| Mode of Delivery | - | |||||||||
| Teaching Methods and Techniques of the Course | DiscussionGroup WorkProblem SolvingCase StudyQ&AApplication: Experiment / Laboratory / WorkshopLecture / Presentation | |||||||||
| Course Coordinator | ||||||||||
| Course Lecturer(s) | ||||||||||
| Assistant(s) | ||||||||||
| Course Objectives | The aim of this course is to teach both the theoretical background and laboratory application principles of the techniques necessary for the processing, modification and artificial transfer of genetic information from cell to cell. In this context, molecular cloning design, use of bacteria and viruses in genetic engineering, expression measurement of artificially transferred genetic material will be taught with modern and conventional techniques. |
| Learning Outcomes |
The students who succeeded in this course;
|
| Course Description | This is a course that explains the systems related to the transfer of theoretical knowledge learned in other Genetics and Bioengineering courses into practice. In this context, the necessary materials and processes in laboratory applications will be explained, and students will be able to both design and implement them. The basic plasmid, bacterial and virus systems used in genetic engineering applications, as well as conventional and modern genetic modification techniques will be explained in detail and with their applications. This course includes basic applications so that students can transfer modern applications of basic experimental design and genetic engineering applications from both academic and industrial perspectives. |
|
|
Core Courses | |
| Major Area Courses |
X
|
|
| Supportive Courses | ||
| Media and Management Skills Courses | ||
| Transferable Skill Courses |
WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES
| Week | Subjects | Related Preparation |
| 1 | Enzymes used in molecular cloning | Kurnaz, Isil Aksan. Techniques in Genetic Engineering: Chapter 1. Plasmids 101 – a desktop resource (3rd edition). Addgene, 2017: Chapter-1 |
| 2 | Plasmids and bacteria used in molecular cloning | Kurnaz, Isil Aksan. Techniques in Genetic Engineering: Chapter 2. Plasmids 101 – a desktop resource (3rd edition). Addgene, 2017: Chapter-1 |
| 3 | Plasmid circuit components and their design and isolation | Kurnaz, Isil Aksan. Techniques in Genetic Engineering: Chapter 2. Plasmids 101 – a desktop resource (3rd edition). Addgene, 2017: Chapter-1 ve 3 |
| 4 | Gene amplification and cutting techniques for cloning | Kurnaz, Isil Aksan. Techniques in Genetic Engineering: Chapter 2. Plasmids 101 – a desktop resource (3rd edition). Addgene, 2017: Chapter-2 |
| 5 | Viral methods in molecular cloning and gene delivery and gene induction | Kurnaz, Isil Aksan. Techniques in Genetic Engineering: Chapter 2 and 4. Plasmids 101 – a desktop resource (3rd edition). Addgene, 2017: Chapter-4 |
| 6 | Recombinant protein labeling methods, reporter systems and purification | Kurnaz, Isil Aksan. Techniques in Genetic Engineering: Chapter 4. Plasmids 101 – a desktop resource (3rd edition). Addgene, 2017: Chapter-6 |
| 7 | Recombinant protein labeling methods, reporter systems and purification | Kurnaz, Isil Aksan. Techniques in Genetic Engineering: Chapter 4 / Plasmids 101 – a desktop resource (3rd edition). Addgene, 2017: Chapter 6 |
| 8 | Midterm | |
| 9 | Real-time PCR and analysis | Kurnaz, Isil Aksan. Techniques in Genetic Engineering: Chapter 1. Vandesompele, Jo, et al. "Real-time PCR: current technology and applications." Reference gene validation software for improved normalization 2 (2009): 47-64. |
| 10 | Mutagenesis | Kurnaz, Isil Aksan. Techniques in Genetic Engineering. Chapter 5 |
| 11 | Mutagenesis and RNA interference | Kurnaz, Isil Aksan. Techniques in Genetic Engineering. Chapter 5 / Martin, Scott E., and Natasha J. Caplen. "Applications of RNA interference in mammalian systems." Annu. Rev. Genomics Hum. Genet. 8 (2007): 81-108. |
| 12 | Conventional gene manipulation | Kurnaz, Isil Aksan. Techniques in Genetic Engineering. Chapter 8. Hoess, R. H., and K. Abremski. "The Cre-lox recombination system." Nucleic Acids and Molecular Biology 4 (1990): 99-109. |
| 13 | Gene editing techniques with CRISPR | Kurnaz, Isil Aksan. Techniques in Genetic Engineering. Chapter 8. Ran, F. Ann, et al. "Genome engineering using the CRISPRCas9 system." Nature protocols 8.11 (2013): 2281-2308. |
| 14 | Gene editing and ethical discussions | Kurnaz, Isil Aksan. Techniques in Genetic Engineering. Chapter 8. Ran, F. Ann, et al. "Genome engineering using the CRISPRCas9 system." Nature protocols 8.11 (2013): 2281-2308. |
| 15 | Semester Review | |
| 16 | Final exam |
| Course Notes/Textbooks | Kurnaz, Isil Aksan. Techniques in Genetic Engineering. 1st ed. CRC Press, 2015. Web. 25 Sept. 2021. ISBN 9780367658816 |
| Suggested Readings/Materials |
|
EVALUATION SYSTEM
| Semester Activities | Number | Weigthing |
| Participation | ||
| Laboratory / Application |
1
|
40
|
| Field Work | ||
| Quizzes / Studio Critiques | ||
| Portfolio | ||
| Homework / Assignments | ||
| Presentation / Jury | ||
| Project | ||
| Seminar / Workshop | ||
| Oral Exams | ||
| Midterm |
1
|
30
|
| Final Exam |
1
|
30
|
| Total |
| Weighting of Semester Activities on the Final Grade |
2
|
70
|
| Weighting of End-of-Semester Activities on the Final Grade |
1
|
30
|
| Total |
ECTS / WORKLOAD TABLE
| Semester Activities | Number | Duration (Hours) | Workload |
|---|---|---|---|
| Theoretical Course Hours (Including exam week: 16 x total hours) |
16
|
3
|
48
|
| Laboratory / Application Hours (Including exam week: '.16.' x total hours) |
16
|
2
|
32
|
| Study Hours Out of Class |
14
|
2
|
28
|
| Field Work |
0
|
||
| Quizzes / Studio Critiques |
0
|
||
| Portfolio |
0
|
||
| Homework / Assignments |
3
|
10
|
30
|
| Presentation / Jury |
0
|
||
| Project |
0
|
||
| Seminar / Workshop |
0
|
||
| Oral Exam |
0
|
||
| Midterms |
1
|
10
|
10
|
| Final Exam |
1
|
32
|
32
|
| Total |
180
|
COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP
|
#
|
Program Competencies/Outcomes |
* Contribution Level
|
||||
|
1
|
2
|
3
|
4
|
5
|
||
| 1 | To have adequate knowledge in Mathematics, Science and Genetics and Bioengineering; to be able to use theoretical and applied information in these areas on complex engineering problems. |
|||||
| 2 | To be able to identify, define, formulate, and solve complex Genetics and Bioengineering problems; to be able to select and apply proper analysis and modeling methods for this purpose. |
X | ||||
| 3 | To be able to design a complex system, process, device or product under realistic constraints and conditions, in such a way as to meet the requirements; to be able to apply modern design methods for this purpose. |
X | ||||
| 4 | To be able to devise, select, and use modern techniques and tools needed for analysis and solution of complex problems in Genetics and Bioengineering applications; to be able to use information technologies effectively. |
X | ||||
| 5 | To be able to design and conduct experiments, gather data, analyze and interpret results for investigating complex engineering problems or Genetics and Bioengineering research topics. |
X | ||||
| 6 | To be able to work efficiently in Genetics and Bioengineering disciplinary and multi-disciplinary teams; to be able to work individually. |
|||||
| 7 | To be able to communicate effectively in Turkish, both orally and in writing; to be able to author and comprehend written reports, to be able to prepare design and implementation reports, to present effectively, to be able to give and receive clear and comprehensible instructions. |
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| 8 | To have knowledge about global and social impact of Genetics and Bioengineering practices on health, environment, and safety; to have knowledge about contemporary issues as they pertain to engineering; to be aware of the legal ramifications of Genetics and Bioengineering solutions. |
|||||
| 9 | To be aware of ethical behavior, professional and ethical responsibility; to have knowledge about standards utilized in Genetics and Bioengineering applications. |
X | ||||
| 10 | To have knowledge about industrial practices such as project management, risk management, and change management; to have awareness of entrepreneurship and innovation; to have knowledge about sustainable development. |
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| 11 | To be able to collect data in the area of Genetics and Bioengineering, and to be able to communicate with colleagues in a foreign language. |
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| 12 | To be able to speak a second foreign language at a medium level of fluency efficiently. |
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| 13 | To recognize the need for lifelong learning; to be able to access information, to be able to stay current with developments in science and technology; to be able to relate the knowledge accumulated throughout the human history to Genetics and Bioengineering. |
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*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest
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