FACULTY OF ENGINEERING

Department of Genetics and Bioengineering

GBE 403 | Course Introduction and Application Information

Course Name
Cell Culture Techniques
Code
Semester
Theory
(hour/week)
Application/Lab
(hour/week)
Local Credits
ECTS
GBE 403
Fall/Spring
2
2
3
6

Prerequisites
None
Course Language
English
Course Type
Elective
Course Level
First Cycle
Mode of Delivery -
Teaching Methods and Techniques of the Course Discussion
Group Work
Q&A
Lecture / Presentation
Course Coordinator
Course Lecturer(s)
Assistant(s) -
Course Objectives The aim of this course is to use cell culture techniques, to be able to work under sterile conditions in cell culture
Learning Outcomes The students who succeeded in this course;
  • Explain different cell culture techniques
  • Work under sterile conditions
  • Describe how cells are cloned, selected and separated
  • Define how cells are differentiated and transformed
  • Discuss how cells are protected from contamination
Course Description Cell culture, Sterile conditions, Primary cell culture, Cell lines, Culture of stem cells and tumor cells, Three-dimensional cell culture

 



Course Category

Core Courses
Major Area Courses
X
Supportive Courses
Media and Management Skills Courses
Transferable Skill Courses

 

WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES

Week Subjects Related Preparation
1 Biology of Cultured Cells Culture of Animal Cells-Chapter 2
2 Laboratory Design, Layout, Equipment and Materials Culture of Animal Cells-Chapter 3 and 4
3 Aseptic Technique, Safety, Bioethics and Validation Culture of Animal Cells-Chapter 5 and 6
4 Culture Vessels and Substrates and Defined and Serum-Free Media Culture of Animal Cells-Chapter 7, 8 and 9
5 Preparation and Sterilization Culture of Animal Cells-Chapter 10
6 Primary Culture Culture of Animal Cells-Chapter 11
7 1.Midterm
8 Subculture and Cell Lines Culture of Animal Cells-Chapter 12
9 Cloning and Selection Culture of Animal Cells-Chapter 13
10 Cell Separation and Characterization Culture of Animal Cells-Chapter 14 and 15
11 Differentiation, Transformation and Immortalization Culture of Animal Cells-Chapter 16 and 17
12 2.Midterm
13 Contamination and Cryopreservation Culture of Animal Cells-Chapter 18 and 19
14 Stem Cells, Germ Cells and Amniocytes Culture of Animal Cells-Chapter 23
15 Culture of Tumor Cells and Three-Dimensional Culture Culture of Animal Cells-Chapter 24 and 25
16 Final exam

 

Course Notes/Textbooks Culture of Animal Cells, A Manual of Basic Technique and Specialized Applications 6th ed. By R. Ian Freshney. John Wiley&Sons (2010).
Suggested Readings/Materials

 

EVALUATION SYSTEM

Semester Activities Number Weigthing
Participation
Laboratory / Application
Field Work
Quizzes / Studio Critiques
2
20
Portfolio
Homework / Assignments
Presentation / Jury
1
10
Project
1
10
Seminar / Workshop
Oral Exams
Midterm
2
30
Final Exam
1
30
Total

Weighting of Semester Activities on the Final Grade
5
70
Weighting of End-of-Semester Activities on the Final Grade
1
30
Total

ECTS / WORKLOAD TABLE

Semester Activities Number Duration (Hours) Workload
Theoretical Course Hours
(Including exam week: 16 x total hours)
16
2
32
Laboratory / Application Hours
(Including exam week: '.16.' x total hours)
16
2
32
Study Hours Out of Class
14
1
14
Field Work
0
Quizzes / Studio Critiques
2
15
30
Portfolio
0
Homework / Assignments
0
Presentation / Jury
1
20
20
Project
1
20
20
Seminar / Workshop
0
Oral Exam
0
Midterms
2
8
16
Final Exam
1
16
16
    Total
180

 

COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP

#
Program Competencies/Outcomes
* Contribution Level
1
2
3
4
5
1

To have adequate knowledge in Mathematics, Science and Genetics and Bioengineering; to be able to use theoretical and applied information in these areas on complex engineering problems.

2

To be able to identify, define, formulate, and solve complex Genetics and Bioengineering problems; to be able to select and apply proper analysis and modeling methods for this purpose.

X
3

To be able to design a complex system, process, device or product under realistic constraints and conditions, in such a way as to meet the requirements; to be able to apply modern design methods for this purpose.

4

To be able to devise, select, and use modern techniques and tools needed for analysis and solution of complex problems in Genetics and Bioengineering applications; to be able to use information technologies effectively.

X
5

To be able to design and conduct experiments, gather data, analyze and interpret results for investigating complex engineering problems or Genetics and Bioengineering research topics.

X
6

To be able to work efficiently in Genetics and Bioengineering disciplinary and multi-disciplinary teams; to be able to work individually.

X
7

To be able to communicate effectively in Turkish, both orally and in writing; to be able to author and comprehend written reports, to be able to prepare design and implementation reports, to present effectively, to be able to give and receive clear and comprehensible instructions.

8

To have knowledge about global and social impact of Genetics and Bioengineering practices on health, environment, and safety; to have knowledge about contemporary issues as they pertain to engineering; to be aware of the legal ramifications of Genetics and Bioengineering solutions.

9

To be aware of ethical behavior, professional and ethical responsibility; to have knowledge about standards utilized in Genetics and Bioengineering applications.

X
10

To have knowledge about industrial practices such as project management, risk management, and change management; to have awareness of entrepreneurship and innovation; to have knowledge about sustainable development.

11

To be able to collect data in the area of Genetics and Bioengineering, and to be able to communicate with colleagues in a foreign language.

12

To be able to speak a second foreign language at a medium level of fluency efficiently.

13

To recognize the need for lifelong learning; to be able to access information, to be able to stay current with developments in science and technology; to be able to relate the knowledge accumulated throughout the human history to Genetics and Bioengineering.

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest

 


NEWS |ALL NEWS

Izmir University of Economics
is an establishment of
izto logo
Izmir Chamber of Commerce Health and Education Foundation.
ieu logo

Sakarya Street No:156
35330 Balçova - İzmir / Turkey

kampus izmir

Follow Us

İEU © All rights reserved.